Autolytic activation mechanism of Bombyx acid cysteine protease (BCP)
نویسندگان
چکیده
منابع مشابه
Mechanism of cysteine protease inactivation by peptidyl epoxides.
Peptidyl epoxides are time- and concentration-dependent selective cysteine protease inhibitors. The lack of recovery of enzymic activity and the retention of 1 molar equivalent of radioactive inhibitor associated with the enzyme on dialysis, shown in this study, indicate that they form a covalent irreversible equimolar complex with the enzyme. It is also shown that the peptidyl epoxide inhibito...
متن کاملProperties of erythrocyte membrane binding and autolytic activation of calcium-activated neutral protease.
The binding of a calcium-activated neutral protease (CANP) with high calcium sensitivity (muCANP) to erythrocyte membranes and its subsequent autolytic activation on the membranes were analyzed by an immunoblot technique. In the presence of calcium ions, muCANP bound to the erythrocyte membranes as a heterodimer of 79- and 28-kDa subunits and was converted quickly on the membranes to an active ...
متن کامل[Activation mechanism of calcium-activated neutral protease].
The activation mechanism through limited autolysis of a calcium-activated neutral protease (CANP) with a high sensitivity to calcium ions (pCANP) was analyzed. The rate of autolysis was dependent on pCANP concentration. The reaction was inhibited by high concentrations of digestible substrates but not by a nondigestible substrate. Incubation of pCANP inactivated by Nethylmaleimide with a small ...
متن کاملEffects of the sintering temperature on the flux-pinning mechanism and the activation energy of malic-acid doped MgB2
The flux-pinning mechanism and activation energy of 10 wt % malic acid-doped MgB2 were investigated by measuring of the critical current density and resistivity as a function of magnetic field and temperature. A crossover field, Bsb, was observed from the single vortex to the small vortex bundle pinning regime. For the sintered sample, the temperature dependence of Bsb(T) at low temperature i...
متن کاملThe Cysteine Protease–Cysteine Protease Inhibitor System Explored in Soybean Nodule Development
Almost all protease families have been associated with plant development, particularly senescence, which is the final developmental stage of every organ before cell death. Proteolysis remobilizes and recycles nitrogen from senescent organs that is required, for example, seed development. Senescence-associated expression of proteases has recently been characterized using large-scale gene express...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 1997
ISSN: 1521-6543
DOI: 10.1080/15216549700203001